Research Article | Published: 01 June 2002

Peroxidase Activity as a Marker of Xylogenesis in the cultured cells of Psidium guajava CV. Navaloor

Ravindra B. Malabadi and K. Nataraja

Indian Journal of Forestry | Volume: 25 | Issue: 2 | Page No. 196-200 | 2002
DOI: https://doi.org/10.54207/bsmps1000-2002-13144G | Cite this article

Abstract

Tracheary element differentiation in calli of different ages of Psidium guajava cv. Navaloor variety has been studied. Hypocotyl explants were cultured on MS (Murashige and Skoog, 1962) medium supplemented with 2, 4-Dichlorophenoxyacetic acid (1 mgl-1: 4.52 µm) and Kinetin (1 mgl-1: 4.65 µm) in combination. Peroxidase activity was measured in respect of age of callus. Present study shows a direct correlation between peroxidise activity and tracheary element differentiation.

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References

1. Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram of protein utilizing the principle of protein dye binding. Anal Biochem., 72:248-254.  https://doi.org/10.1006/abio.1976.9999

Google Scholar

2. Brett and Waldron, K. (1990). Control of cell wall extensibility. In: Black, M. and Chapman, J. (eds.) Physiology and Biochemistry of Plant Cell Walls (pp. 101-103). Unwin Hyman. London.

Google Scholar

3. Bruce, R.J. and West, C.A. (1989). Eliciation of lignin Biosynthesis and isoperoxidase activity by pectic fragments in suspension cultures of Caster Bean. Plant Physiology, 889-897.  https://doi.org/10.1104/pp.91.3.889

Google Scholar

4. Egley, G.H.; Paul, R.N. Jr; Duke, S.O. and Vaughn, K.C. (1985). Peroxidase involvement in lignifications in water impermeable seed coats of weedy leguminous and malvaceous species. Plant Cell Environ., 8:253-260.  https://doi.org/10.1111/j.1365-3040.1985.tb01397.x

Google Scholar

5. Fry, S.C. (1986). Cross linking of martrix polymeres in the growing Cell – Walls of angiosperms. Annu. Rev. Plant Physiol., 37:165-186.  https://doi.org/10.1146/annurev.pp.37.060186.001121

Google Scholar

6. Gasper, T.H.; Penel, C.; Thorpe, T. and Greppin, H. (1982). Peroxidases. A survey of their biochemical and physiological roles in higher plants. Universito de Geneva, Geneva.

Google Scholar

7. Gasper, T.; Penel, C.; Hagege, D. and Greppin, H. (1991). Peroxidases in plant growth, differentiation and development processes. In: Labarzewsky, J.; Greppin, H.; Penel, C. and Gasper, T. (eds.) Biochemical Molecular and Physiological aspects of plant peroxidises.

Google Scholar

8. Gazaryan, I.G.; Urmansteva, V.V.; Veryovkin, A.N. and Fechina, V.A. (1991). Peroxidase preparation from Medicago sativa L-1 Cell culture. In: Lobarzewski, J.; Greppin, H.; Penel, C. and Gasper, t. (eds.) Biochemical Molecular and Physiological aspects of plant peroxidises (pp. 505-506). University of Geneva, Switzerland.

Google Scholar

9. Goldberge, R.; Imberty, A.; Liberman, M. and Prat, R. (1986). Relationship between peroxidise activities and cell-wall plasticity. In: Greppin, H.; Penel, C. and Gasper, T. (eds.) Molecular and Physiological aspects of plant peroxidises (pp. 209-220). Univ. Geneva, Switzerland.

Google Scholar

10. Grisebach, H. (1977). Biochemistry of lignifications. Naturwissenschaften., 64:619-625.  https://doi.org/10.1007/BF00440094

Google Scholar

11. Higuchi, T. (1985). Biosynthesis of lignin. In: T. Higuchi (ed.) Biosynthesis and Biodegradation of wood components. Academic Press, New York. Pp. 141-160.  https://doi.org/10.1016/B978-0-12-347880-1.50011-8

Google Scholar

12. Kim, S.H.; Terry, M.E.; Hoops, P.; Dauwalder, M. and Roux, S.J. (1988). Production and Characterization of monoclonal antibodies to wall localized peroxidises from corm seedlings. Plant Physiol., 88:1446-1453.  https://doi.org/10.1104/pp.88.4.1446

Google Scholar

13. Lagrimini, L.M.; Burkhart, W.; Moyer, M. and Rothstein, S. (1987). Molecular cloning of complementary DNA encoding the lignin forming Peroxidase from tobacco: Molecular analysis and tissue specific expression. Proc. Natl. Acad. Aci. USA, 84:7542-7546.  https://doi.org/10.1073/pnas.84.21.7542

Google Scholar

14. Legrand, B. and Dubois, J. (1977). Evolution des peroxidises et auxinesxydases au cours de la croissance dune suspension cellulaire de silene (Silene alba (Miller) E. A.L. Krause). CR. Acad. Sc. Paris. 285:661-664.

Google Scholar

15. Lamport, D.T.A. (1986). Roles of peroxidises in cell wall genesis. In: Greppin, H.; Penel, C. and Gasper, T. (eds.) Molecular and Physiological Aspects of Plant peroxidises (pp. 199-208). Univ. Geneva, Switzerland.

16. Lewis, N.G. and Yamamoto, E. (1990). Lignin: Occurrence Biogenesis and biodegradation. Ann. Rev. Plant Physiol. Plant Mol. Biol., 41:455-496.  https://doi.org/10.1146/annurev.pp.41.060190.002323

Google Scholar

17. Macek, T.; Dobransky, T.; Pospisilova, R.; Vanek, T. and Kralova, B. (1993). Peroxidases from in vitro cultures of different plant species In: Welinder, K.G.; Rasmussen, S.K.; Penel, C. and Greppin, H. (eds.) Plant peroxidises: Biochemistry and Physiology (pp. 451-455). University of Geneva.

Google Scholar

18. Mizuno and Komamine, A. (1978). Isolation and Identification of substances inducing formation of Tracheary elements in cultured carrot root slices. Planta, 138:59-62.  https://doi.org/10.1007/BF00392915

Google Scholar

19. Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant, 15:473-497.  https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Google Scholar

20. Perrey, R.; Hauser, M.T. and Wink, M. (1980). Cellular and subcellular localization of peroxidises isoenzymes in plant and cell suspension cultures from Lupinus polyphyllus. Z. Natuv.

Google Scholar

21. Putter, J. (1974). In: Methods of Enzymatic analysis 2 (Ed. Bergmcyer). Academic Press, New York. P. 685.

Google Scholar

22. Ryugo, K. (1962). The accumulation of lignin and the concurrent changes in the apparent density of the cell-wall in the peach endocarp. J. Am. Soc. Hortic. Sci., 80:197-203.

Google Scholar

23. Sadasivam, S. and Manickam, A. (1996). Biochemical Methods (Second edition). New Age International (P) Ltd. Publishers (pp. 108-110).

Google Scholar

24. Shailaja, R.; Chennaveeraiah, M.S. and Nataraja, K. (1983). Effect of Indole-3-Acetic-Acid on Phenolic Accumulation & Peroxidase Activity in Cell Cultures of Mussenda rosea L. Indian Journal of Experimental Biology, 21:653-655.

Google Scholar

25. Sherf, B.A. and Kolattukudy, P.E. (1993). Developmentally regulated expression of the wound and pathogen responsive tomato anionic peroxidises in green fruit. Plant. J., 3:829-833.  https://doi.org/10.1046/j.1365-313X.1993.03060829.x

Google Scholar

26. Vance, C.P.; Anderson, J.O. and Sherwood, R.T. (1976). Soluble and cell wall peroxidises in reed canary grass in relation to disease resistance and localized lignin formation. Plant Physiol., 57:920-922.  https://doi.org/10.1104/pp.57.6.920

Google Scholar

27. Van Huystee, R.B.; Breda, C.; Sesto, P.; Beopouolos, N. and Esnault, R. (1990). Measurement and detection of peroxidises. Plant Sci., 69:19-26.  https://doi.org/10.1016/0168-9452(90)90100-3

Google Scholar

28. Whitemore, F.W. (1978). Lignin – Carbohydrate complex formed in isolated cell walls of callus. Phytochem., 17: 421-425.  https://doi.org/10.1016/S0031-9422(00)89329-2

Google Scholar

29. Worthington Enzyme Manual (1972). Enzymes. Enzyme reagents Related Biochemicals. Worthington Biochemical Corporation, Freehold, New Jersey, USA pp. 43-45.

Google Scholar

30. Zheng, X. and Van Huystee, R.B. (1992). Peroxidase regulated elongation of segments from peanut hypocotyls. Plant Sci., 81:47-56.  https://doi.org/10.1016/0168-9452(92)90023-F

Google Scholar

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How to cite

Malabadi, R.B. and Nataraja, K., 2002. Peroxidase Activity as a Marker of Xylogenesis in the cultured cells of Psidium guajava CV. Navaloor. Indian Journal of Forestry, 25(2), pp.196-200. https://doi.org/10.54207/bsmps1000-2002-13144G

Publication History

Manuscript Published on 01 June 2002

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