Abstract
The combination of different concentration of substrate (0.10M, 0.15M, 0.20 and 0.25M; KNO3) with different pH of buffer (0.2M KH2PO4 of the pH 6.5, 7.0, 7.5, 7.6, 7.7, 7.8, 7.9, 8.0, 8.5 and 9.0) solution were tried for the nitrate reductase activity of Mimosa hamata Willd. leaves. Maximum nitrate reductase activity was observed in the combination of buffer solution of 0.20 M having pH 7.7 and substrate solution of the concentration 0.20 M.
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