Research Article | Published: 01 December 2015

Standardization of substrate and buffer concentrations for in-vivo nitrate reductase activity in Adenanthera microsperma leaves

Priyanshu Sharma, S. P. Chaukiyal and Meenu  Singh  Sengar

Indian Journal of Forestry | Volume: 38 | Issue: 4 | Page No. 309-311 | 2015
DOI: https://doi.org/10.54207/bsmps1000-2015-5DBCB9 | Cite this article

Abstract

The combination of different substrate concentrations (0.05M, 0.10M, 0.15M, 0.20M and 0.25M, KNO3) with different pH of phosphate buffer (0.10 M and 0.20 M, KH2PO4 of the pH 7.0, 7.5, 7.6, 7.7, and 7.8) solutions were tried for  in-vivo nitrate reductase activity of Adenanthera microsperma leaves. Maximum nitrate reductase activity was observed in the combination of buffer solution (0.20M KH2PO4) having pH 7.7 and substrate solution 0.20 M concentration.

Keywords

Adenanthra microsperma; Buffer; Nitrate Reductase Activity; Substrate

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How to cite

Sharma, P., Chaukiyal, S.P. and Sengar, M.S., 2015. Standardization of substrate and buffer concentrations for in-vivo nitrate reductase activity in Adenanthera microsperma leaves. Indian Journal of Forestry, 38(4), pp.309-311. https://doi.org/10.54207/bsmps1000-2015-5DBCB9

Publication History

Manuscript Published on 01 December 2015

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