Research Article | Published: 01 March 2009

Effect of different media compositions on asymbiotic germination of Cymbidiums

Seventhilingam Kaliamoorthy and G. V. S. Murthy

Indian Journal of Forestry | Volume: 32 | Issue: 1 | Page No. 75-80 | 2009
DOI: https://doi.org/10.54207/bsmps1000-2009-727DF3 | Cite this article

Abstract

A protocol for seed germination and seedling culture of Cymbidium aloifolium (L.) Sw. and C. bicolor Lindl. Was developed. Green “matured” capsules containing seeds were surface sterilized and cultured on various media compositions. Murashige and Skoog (MS) medium with or without 10% coconut water (CW) promoted higher rate of initial seed germination. This was followed by modified Knudson’s C and Phytamax media supplemented with or without 10% CW. Further studies on seedling multiplication revealed that MS medium supplemented with 10% CW was more effective in axillary shoot proliferation. Rooted seedlings were successfully acclimatized to the greenhouse environment. Results of this study demonstrate that Cymbidiums can be successfully propagated through asymbiotic germination method for possible use in re-introduction in situ and conservation projects.

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Fig. 1

Fig. 1: 45 days old seed cultures of Cymbidium bicolor showing clumps of spherical shaped PLBs. Scale bar = 0.5 cm.
Fig. 2: Uniformly grown PLBs showing shoot development. Note the shoots with prominent leaves. Scale bar = 0.5 cm.
Fig. 3: Stages of development in Cumbidium aloifolium. Note the multiplication of axillary shoots from the base. Scale bar = 1.0 cm.
Fig. 4: Shoot multiplication with root development in C. bicolor. Scale bar = 1.0 cm.
Fig. 5: Uniformly well-developed seedlings of C. bicolor ready for transfer to community pots. Scale bar = 1.0 cm.
Fig. 6: Seedlings growing well in the community pot containing brick pieces: charcoal (1:1) Scale bar = 1.0 cm.

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How to cite

Kaliamoorthy, S. and Murthy, G.V.S., 2009. Effect of different media compositions on asymbiotic germination of Cymbidiums. Indian Journal of Forestry, 32(1), pp.75-80. https://doi.org/10.54207/bsmps1000-2009-727DF3

Publication History

Manuscript Published on 01 March 2009

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