Research Article | Published: 01 June 2016

Standardization of Suitable Substrate and Buffer Solutions for Optimum in-vivo Nitrate Reductase Activity in the Leaves of Sesbania rostrata Bremek. and Oberm.

S. P. Chaukiyal, Vandana and Poornima  Uniyal

Indian Journal of Forestry | Volume: 39 | Issue: 2 | Page No. 121-124 | 2016
DOI: https://doi.org/10.54207/bsmps1000-2016-7660V8 | Cite this article

Abstract

Sesbania rostrata Bremek. and Oberm. is a multipurpose and exceptionally fast growing nitrogen fixing plant. In this study a protocol for the standardization of in-vivo nitrate reductase (NR) assay with respect to substrate concentrations along with buffer pH and concentrations was carried out in the leaves of S. rostrata. Different substrate (KNO3) concentration ( i.e. 0.025M, 0.05M, 0.10M, 0.15M, 0.20M ) were tried against phosphate buffer (KH2PO4) solutions with different pH (6.5, 7.0, 7.5, 7.6, 7.7, 7.8 ). Maximum leaf in-vivo nitrate reductase activity was observed in 0.10 M phosphate buffer (KH2PO4) concentration of pH 7.5 with 0.15M substrate (KNO3) concentration.

Keywords

Sesbania rostrata, In-vivo Nitrate Reductase (NR) Activity, Substrate and Buffer Solution

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How to cite

Chaukiyal, S.P., Vandana and Uniyal, P., 2016. Standardization of Suitable Substrate and Buffer Solutions for Optimum in-vivo Nitrate Reductase Activity in the Leaves of Sesbania rostrata Bremek. and Oberm.. Indian Journal of Forestry, 39(2), pp.121-124. https://doi.org/10.54207/bsmps1000-2016-7660V8

Publication History

Manuscript Published on 01 June 2016

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