One of the most important steps involved in in-vitro propagation is establishment of contamination free cultures. To overcome the problem of contamination of explants of Dalbergia sissoo Roxb. collected from mature trees present investigation was initiated and it was observed that treatment of explant with 1.10% HgCl2 for 15 minutes was found to be the best and most effective and resulted in establishment of contamination free healthy cultures of Dalbergia sissoo.
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Manuscript Published on 01 December 2002
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