Research Article | Published: 01 June 2013

Assessment of Nitrate Reductase Activity in the Leaves of Erythrina Blakei R. Parker

Abhinay  Ratrey, S. P. Chaukiyal, Pallavi  Bhatia, Neelam and Navjot  Kaur  Deol

Indian Journal of Forestry | Volume: 36 | Issue: 2 | Page No. 191-196 | 2013
DOI: https://doi.org/10.54207/bsmps1000-2013-HSW55X | Cite this article

Abstract

Combinations of different concentrations of substrate (0.025M, 0.05M, 0.1M, 0.15M, 0.20M and 0.25M KNO3) with different pH of buffer (0.1M, KH2PO4 of the pH 6.5, 6.6, 6.7, 6.8, 6.9, 7.0, 7.5, 7.6, 7.7, 7.8) solutions were tried for the nitrate reductase activity of Erythrina blakei leaves. Maximum nitrate reductase activity was observed in the combination of buffer solution of 0.1M having pH 6.8 and substrate solution of the concentration 0.15M. NR activity and moisture content in single leaf blades of Erythrina blakei leaves of different branches from different levels of tree have different concentration of activity and Moisture percentage. In single leaf blades maximum NR activity was recorded in leaf number 10, Branch number 3. And maximum moisture percentage observed in leaf number 12, Branch number 3.

Keywords

Anti-inflammatory, Substrate, Buffers, Assimilation, Physiological

Access Options

250/-

Buy Full Access in HTML Format

Instant access to the full article.

Get access to the full version of this article. Buy Full Access in HTML Format

References

1. Chaukiyal, S.P. (2008a). Standardization of in-vivo nitrate reductase activity in Clitoria ternatea leaves. Indian For. 134 (2): 250-254

Google Scholar

2. Chaukiyal, S.P. (2008b). Standardization of in-vivo nitrate reductase activity in Mucuna   pruriens (L.) DC. Leaves. Ann. For. 134(8): 1067-1070

Google Scholar

3. Chaukiyal, S.P. (2008c). Standardization of in-vivo nitrate reductase activity in Rhynchsia minima (L.) DC. Leaves. Indian For. 16 (2): 251-254

Google Scholar

4. Chaukiyal, S.P. (2008d). Standardization of in-vivo nitrate reductase activity in Crotalaria burhia leaves. Indian For. 135 (7): 965-969

Google Scholar

5. Chaukiyal, S.P. (2008e). Standardization of in-vivo nitrate reductase activity in Mimosa hamata Willd. leaves. Ind. J. For. 32 (2): 239-242

Google Scholar

6. Chaukiyal, S.P. and Mir, R.A. (2010).Assessment of nitrate reductase activity in the leaves of Terminalia chebula. Indian For. 136(9): 1213-1217

Google Scholar

7. Chaukiyal, S.P., Khatri, N., Bhatia, P. and Pokhriyal, T.C. (2012). Standardization of in-vivo nitrate reductase activity and its pattern in the individual leaf blades of Myrica sculenta Buch. Ham. ex D.Don. (under publication).

Google Scholar

8. Chaukiyal, S.P. and Pokhriyal, T.C. (1996). Nitrate assimilation activity and growth pattern in Pongamia pinnata Pierre leaf. Ann. For. 4 (1): 94-100

Google Scholar

9. Evans, H.J. and Nason, A. (1953). Pyridine nucleotide nitrate reductase from extracts of higher plants. Pl. Physiology, 28: 233-254  https://doi.org/10.1104/pp.28.2.233

Google Scholar

10. Klepper, L.D., Flesher, D. and. Hageman, R.H. (1971). Generation of reduced nicotinamide adenine dinucleotide for nitrate reduction in green leaves. Pl. Physiology, 48: 580-590.  https://doi.org/10.1104/pp.48.5.580

Google Scholar

11. Laurie, S and Stewart, G.R. (1993). Effects of nitrogen supply and high temperature on the growth and physiology of Chickpea. Pl. and Cell Environ. 16: 609-621.   https://doi.org/10.1111/j.1365-3040.1993.tb00479.x

Google Scholar

12. Li, X-Z and Oaks, A. (1995). Effect of light on the nitrate and nitrite reductase in Zea mays. Pl. Sci. 109: 115-118  https://doi.org/10.1016/0168-9452(95)04159-R

Google Scholar

13. Nair, T.V.R. and Abrol, Y.P. (1977). Studies of nitrate reducing systems in developing wheat ears. Crop Sci. 17: 428-442  https://doi.org/10.2135/cropsci1977.0011183X001700030024x

Google Scholar

14. Pokhriyal, T.C. and Raturi, A.S. (1984). Nitrate assimilation in leaf blade of Eucalyptus. Indian For., 110(2): 202-208

Google Scholar

15. Pokhriyal, T.C. and Raturi, A.S. (1985). A study of nitrate reductase activity in Populus deltoids leaves. Indian For., 111(2): 82-89

Google Scholar

16. Pokhriyal, T.C. and Raturi, A.S. (1986). Nitrate assimilation in Leucaena leucocephala leaves. Indian For., 112(11): 993-997

Google Scholar

17. Pokhriyal, T.C. and Raturi, A.S. (1988). Standardization of nitrate reductase activity in Albizia lebbeck, Acacia nilotica and Dalbergia sissoo. Indian For. 114(3): Research Notes (I), 166-167

Google Scholar

18. Rautela, P.S., Semwal, P. and Chaukiyal, S.P. (2012). Nitrate reductase activity in the leaf blade of Castanospermum austral. Indian For. as short notes (Communicated).

Google Scholar

19. Schrader, L.E., Cataldo, D.A., Peterson, D.M. and Vogelzang, R.D. (1974). Nitrate reductase and glucose 6 phosphate dehydrogenase activites as influenced by leaf ageand addition of protein to extraction media. Physiologia Plantarum, 32: 337-341.  https://doi.org/10.1111/j.1399-3054.1974.tb03147.x

Google Scholar

20. Semwal, P. Rautela, P.S. and Chaukiyal, S.P. (2012). Nitrate reductase activity in the leaf blade of Grewia optiva (Bhimal) (In Press Ann. For.).

Google Scholar

21. Wallace, W. and Pate, J.S. (1965). Nitrate reductase in the leaf of field pea (Pisum ravens L.) Ann. Bot. 29: 656-671  https://doi.org/10.1093/oxfordjournals.aob.a083979

About this article

How to cite

Ratrey, A., Chaukiyal, S.P., Bhatia, P., Neelam and Deol, N.K., 2013. Assessment of Nitrate Reductase Activity in the Leaves of Erythrina Blakei R. Parker. Indian Journal of Forestry, 36(2), pp.191-196. https://doi.org/10.54207/bsmps1000-2013-HSW55X

Publication History

Manuscript Published on 01 June 2013

Share this article

Anyone you share the following link with will be able to read this content: