Research Article | Published: 01 December 2014

Micropropagation of Plumbago Zeylanica L. through Axillary Bud Culture

J.M.S.  Chauhan and Prabha Bisht

Indian Journal of Forestry | Volume: 37 | Issue: 4 | Page No. 383-386 | 2014
DOI: https://doi.org/10.54207/bsmps1000-2014-R6Y9MT | Cite this article

Abstract

Plumbago zeylanica is a perennial herb or straggling shrub and is greatly valued in ayurvedic treatment of cough, asthma and gastrointestinal disorders. Propagation of the species through seed is unreliable due to poor seed quality, erratic germination and seedling mortality under natural field conditions. Hence, it’s Propagation through tissue culture was sought. The nodal explants were taken as the starting material for the establishment of cultures. After washing the explants were surface sterilized by different sterilants viz. HgCl2, NaOCl and H2O2 with different concentrations and time duration where 0.1% of HgCl2 for 5 minutes was found the best. After sterilization nodal segments were inoculated on MS medium with different hormonal concentrations under aseptic conditions. Bud break was observed after two weeks of culture in 1.0 mg/l of BAP. These axillary shoots were excised and transferred to MS medium supplemented with different hormonal concentrations. A very high rate of multiplication occurred on MS + BAP (1.0 mg/l) + KIN (0.4 mg/l) and NAA (0.2 mg/l). In vitro grown shoots were rooted in ½ strength MS medium supplemented with different concentration of IBA (0.5, 1.0, 1.5 mg/l), IAA (0.5, 1.0, 1.5 mg/l). Maximum rooting was recorded in 0.5 mg/l IBA. After one month on rooting medium healthy roots were developed and there after plantlets were transferred to mist chamber for acclimatization.

Keywords

Perennial herb, Ayurvedic treatment, Propagation, Hormonal concentrations, Tissue culture, Sterilization, Culture medium

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How to cite

Chauhan, J. and Bisht, P., 2014. Micropropagation of Plumbago Zeylanica L. through Axillary Bud Culture. Indian Journal of Forestry, 37(4), pp.383-386. https://doi.org/10.54207/bsmps1000-2014-R6Y9MT

Publication History

Manuscript Published on 01 December 2014

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