Research Article | Published: 01 December 2011

Micropropagation of Alnus nitida (Endl.): a novel technique for its conservation

Syed Arshad Hussain, Azra N. Kamili and A. M. Shah

Indian Journal of Forestry | Volume: 34 | Issue: 4 | Page No. 415-422 | 2011
DOI: https://doi.org/10.54207/bsmps1000-2011-T585I8 | Cite this article

Abstract

To conserve Alnus nitida species a micropropagation technique was developed using shoot apices/nodal stem segments as starting material for establishment of axenic shoot cultures on cytokinin supplemented MS medium. The established shoots were transformed into multiple shoot cultures on a multiplication medium consisting of MS/B5 medium augmented with a combination of BA/Kn (2.5-10 µM) and IAA/NAA/IBA (2 µM). A combination of BA (5 µM) and NAA (2 µM) in MS medium lead to formation of a maximum 32.2±0.6 shoots/explant after 8 weeks culture period. The sufficiently elongated microshoots were later on rooted on an auxin enriched half strength MS medium. The microplants were demonstrated to show 70% survival upon being transferred to small pots containing vermiculite sand mixture.

Keywords

Alder, Vulnerable, Labolene, Auxin, Nodal Segment, Degree of callus formation

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How to cite

Hussain, S.A., Kamili, A.N. and Shah, A.M., 2011. Micropropagation of Alnus nitida (Endl.): a novel technique for its conservation. Indian Journal of Forestry, 34(4), pp.415-422. https://doi.org/10.54207/bsmps1000-2011-T585I8

Publication History

Manuscript Published on 01 December 2011

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