Research Article | Published: 22 March 2017

Extraction of Genomic DNA from Inner Bark of Cedrus deodara (Roxb.) G. Don for RAPD Study

Akhilesh  Kumar, Santan Barthwal and Harish Singh Ginwal

Indian Journal of Forestry | Volume: 40 | Issue: 1 | Page No. 25-27 | 2017
DOI: https://doi.org/10.54207/bsmps1000-2017-UD2117 | Cite this article

Abstract

Genomic DNA extraction from forestry tree species require young leaf samples to obtain high-quality DNA for molecular based study. For some study, leaf samples must be collected from remote areas and are difficult to transport long distances. We developed alternative method of DNA extraction from inner bark of Cedrus deodara. We used 2.5% PVP for removal of phenolic compound and apply high concentration of sodium chloride to removes polysaccharides. Extracted DNA gives positive amplification with PCR using random amplification of polymorphic DNA (RAPD) markers.

Keywords

Genomic DNA, RAPD, PCR, Cedrus deodara, Inner Bark, PVP

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How to cite

Kumar, A., Barthwal, S. and Ginwal, H.S., 2017. Extraction of Genomic DNA from Inner Bark of Cedrus deodara (Roxb.) G. Don for RAPD Study. Indian Journal of Forestry, 40(1), pp.25-27. https://doi.org/10.54207/bsmps1000-2017-UD2117

Publication History

Manuscript Published on 22 March 2017

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