Research Article | Published: 22 June 2017

Copper and Zinc Induced Amelioration of In vitro Multiplication of Dendrocalamus strictus (Roxb.) Nees

Manish  Singh, Manpreet Kaur, Meena Bakshi, Satakshi  Kapurwan and Abhishek  Kumar

Indian Journal of Forestry | Volume: 40 | Issue: 2 | Page No. 181-184 | 2017
DOI: https://doi.org/10.54207/bsmps1000-2017-VV3901 | Cite this article

Abstract

Bamboos are among most significant plants worldwide and economically important forest resource in many countries of Asia. In vitro propagation is a potential and well known technique for mass multiplication of bamboo. Efforts were made for multiplication of Dendrocalamus strictus through aseptic nodal explants and its acceleration through heavy metal infusion. A rapid in vitro propagation protocol was established. Maximum germination with 100% survival rate was recorded in MS media supplemented with 1.0 mg/l BAP in combination with TDZ (0.25 mg/l) with a maximum shoot number of 9.36±0.67 and shoot length of 6.85 ± 0.95. The effects of copper and zinc were assessed on morphogenesis.  The specific concentration of copper and zinc in MS medium were doubled (2x), quintuple pentadrupled (5x) and decupled (10x). The results revealed that maximum mean shoot number and mean shoot length was observed in quintuple pentadrupled zinc i.e. 6.80 ± 0.74 and 6.60 ± 0.80 cm respectively, however as concerned to copper, maximum shoot number (5.56 ±0.92) and shoot length (5.87 ± 0.78) were observed at similar concentration. Although copper and zinc are toxic for plants at higher concentration, but a good in vitro propagation response was observed in quintuple pentadrupled concentration.

Keywords

In vitro Propagation, Zinc, Copper, Dendrocalamus strictus

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How to cite

Singh, M., Kaur, M., Bakshi, M., Kapurwan, S. and Kumar, A., 2017. Copper and Zinc Induced Amelioration of In vitro Multiplication of Dendrocalamus strictus (Roxb.) Nees. Indian Journal of Forestry, 40(2), pp.181-184. https://doi.org/10.54207/bsmps1000-2017-VV3901

Publication History

Manuscript Published on 22 June 2017

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