Abstract

In vitro plantlet production of Celastrus paniculatus Willd. was established using nodal segment as explants. The maximum shoot establishment was observed on the medium MS + 2 mglˉ¹ BA+ 2 mglˉ¹ Kin + 1 mglˉ¹ NAA. A wide range of variability was observed for per cent shoot establishment given by the two different genotypes of Celastrus paniculatus Wild viz., Dapoli-local and Talavali local. The maximum shoot establishment was observed in genotype Dapoli local and media combination of MS+ 2mglˉ¹ BA+2 mglˉ¹ Kin+ 1 mgˉ¹ NAA and MS+1 mglˉ¹ BA+ 1 mglˉ¹ Kin+ 0.5 mglˉ¹ NAA for Talavali local. The response for days to shoot initiation varied in the both genotypes. The genotype Talavali local had given the best response by taking minimum days to shoot initiation (8.89). Among the various media combinations MS+ 2 mgl ˉ¹ BA+ 2 mgl ˉ¹ Kin+ 1 mgl ˉ¹ NAA medium found to be better in regards to days to shoot initiation with average 9.43 days. The multiple shoot formation rate showed considerable variability between the different genotypes. The genotype Dapoli local (7.91) have higher shoots than Talayali local (6.92). Both genotypes more number of shoots on medium MS +2 mgl ˉ¹ BA+ 2 mgl ˉ¹ Kin +1 mgl ˉ¹ NAA. Among the genetic parameters studied, the magnitude of genotypic variance was greater than environmental variance for all the characters studied. The highest estimates for genotypic and phenotypic variance were found in shoot establishment. The phenotypic coefficient of variation (PCV) was higher than genotypic coefficient of variation (GCV) for all the characters studied. The highest estimates of GCV and PCV were observed in shoot multiplication rate. Heritability was almost same for all characters studied. Genetic advance was higher for character shoot establishment followed by days to first shoot initiation.

Keywords