Research Article | Published: 15 January 2022

In vitro propagation of Asplenium aethiopicum through tissue culture

M. Shunmuga Sundari, Asir Benniamin, Doss Jesubalan and Kaushik Sarkar

Journal of Non-Timber Forest Products | Volume: 28 | Issue: 2 | Page No. 39-42 | 2021
DOI: https://doi.org/10.54207/bsmps2000-2022-21A0H6 | Cite this article

Abstract

Poor viability of spores and germination and slow growth in conventional vegetative propagation are major constraints in the in vitro culture of Asplenium aethiopicum, an important medicinal fern. In the present study, a procedure for in vitro propagation of this endangered fern through croziers has been established. Maximum sporophytes efficiency was obtained on KC media supplemented with growth regulators such as 0.5 mg/l NAA & 0.5 mg/l 2,4-D with 2 sucrose under photoperiod condition. Maximum frequency (33.2%) of callus formation and germination of sporophyte was observed on KC medium with growth regulators.

Keywords

Callus, Ferns, Regeneration, Sporophyte

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How to cite

Sundari, M.S., Benniamin, A., Jesubalan, D. and Sarkar, K., 2021. In vitro propagation of Asplenium aethiopicum through tissue culture. Journal of Non-Timber Forest Products, 28(2), pp.39-42. https://doi.org/10.54207/bsmps2000-2022-21A0H6

Publication History

Manuscript Received on 12 December 2021

Manuscript Revised on 29 December 2021

Manuscript Accepted on 31 December 2021

Manuscript Published on 15 January 2022

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