Research Article | Published: 01 March 2005

Micropropagation of embelia ribes burm a threatened medicinal plant

K. Shankarmurthy, V. Krishna and S. M. Vidya

Journal of Non-Timber Forest Products | Volume: 12 | Issue: 1 | Page No. 18-21 | 2005
DOI: https://doi.org/10.54207/bsmps2000-2005-21DWM2 | Cite this article

Abstract

An efficient micropropagation protocol was developed for the woody medicinal shrub Embelia ribes using stem explant. The nodal and inter nodal segments proliferate in to luxuriant mass of callus on MS medium supplemented with 3 mg/l 2,4-D and 0.6 mg/l Kn. High frequency shoot and root differentiation was achieved in a single phase culture and was optimized on the medium fortified with 2.5 mg/l BAP and 0.7 mg/l NAA. A mean of 86.1±1.66 shoots were differentiated per callus mass and a mean of 68.4±1.71 plantlets were recovered from 60 days incubated culture. Regenerants plants were successfully acclimatized and 94% of plantlets were survived under ex vitro condition, exhibiting a normal development.

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How to cite

Shankarmurthy, K., Krishna, V. and Vidya, S.M., 2005. Micropropagation of embelia ribes burm a threatened medicinal plant. Journal of Non-Timber Forest Products, 12(1), pp.18-21. https://doi.org/10.54207/bsmps2000-2005-21DWM2

Publication History

Manuscript Published on 01 March 2005

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