Research Article | Published: 01 September 2011

Genetic Diversity Assessment of Underexploited Legume of Mucuna Atropurpurea DC. in Southern Western Ghats, Tamil Nadu

Chinnamadasamy Kalidass and V. R. Mohan

Journal of Non-Timber Forest Products | Volume: 18 | Issue: 3 | Page No. 195-200 | 2011
DOI: https://doi.org/10.54207/bsmps2000-2011-395712 | Cite this article

Abstract

In the present investigations, Randomly Amplified Polymorphic DNA (RAPD) technique has been used to detect polymorphism of Mucuna atropurpurea DC. in Southern Western Ghats, Tamil Nadu, India. The genomic DNA was extracted from the fresh and young healthy leaves of the selected species using modified CTAB method which successfully produced a good purity and quality of DNA. Alleles of various sizes ranging from 100 – 800bp were amplified. The average genetic distance range was 67% – 91%. Phylogenetic relationships among these accessions based upon DNA analysis were studied through dendrogram analysis. All the accessions were clustered in to two groups. In this cluster the Kothaiyar and Saduragiri accessions were found most distantly related to each other.

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Fig. 1

Map showing the distributions of Mucuna atropurpurea DC. and locations of seven accessions samples from Southern Western Ghats, Tamil Nadu, India

Fig. 2

RAPD amplification profile of Mucuna atropurpurea DC. with different primers. A. Primer OPE-11; B. OPA-02; C. OPH-17; D. OPAC-02; E. OPA-03. Lines (1 to 8); M-Market 100bp DNA ladder; 1. Sivagiri; 2. Thallaianai; 3. Kothaiyar; 4. Saduragiri; 5. Ayyanarkovil; 6. Anaikatti; 7. Aliyar

Fig. 3

UPGMA dendrogram based on Nei and Li (1979) genetic difference between seven Mucuna atropurpurea DC. accessions according to RAPD analysis

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How to cite

Kalidass, C. and Mohan, V.R., 2011. Genetic Diversity Assessment of Underexploited Legume of Mucuna Atropurpurea DC. in Southern Western Ghats, Tamil Nadu. Journal of Non-Timber Forest Products, 18(3), pp.195-200. https://doi.org/10.54207/bsmps2000-2011-395712

Publication History

Manuscript Published on 01 September 2011

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