Research Article | Published: 01 December 2009

Clonal propagation of Tylophora subramanii A.N.Henry in Agasthiyamalai Biosphere Reserve in India

A. John De Britto and R. Mahesh

Journal of Non-Timber Forest Products | Volume: 16 | Issue: 4 | Page No. 347-350 | 2009
DOI: https://doi.org/10.54207/bsmps2000-2009-7ZMVN7 | Cite this article

Abstract

Tissue culture system was established from shoot tips of the Tylophora subramanii A.N.Henry. Shoot proliferation was achieved with MS (Murashige and Skoog) medium supplemented with KI (Kinetin) with different concentrations. Stocking of shoot cultures was obtained by repeating subculture in the same medium at 40 day intervals wherein the rate of multiplication was maintained. For rooting the individuals shoot was implanted on root induction medium consisted of MS medium supplemented with 0.4 mg/l NAA. 1-naphthaleneacetic acid. Within two weeks of incubation 70% rooting was evidenced. Complete plantlets were acclimatized and successfully transplanted to natural habitats.

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How to cite

De Britto, A.J. and Mahesh, R., 2009. Clonal propagation of Tylophora subramanii A.N.Henry in Agasthiyamalai Biosphere Reserve in India. Journal of Non-Timber Forest Products, 16(4), pp.347-350. https://doi.org/10.54207/bsmps2000-2009-7ZMVN7

Publication History

Manuscript Published on 01 December 2009

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