Research Article | Published: 24 September 2021

Identification of female sex-specific DNA marker in a dioecious rattan (Calamus travancoricus Bedd. ex Becc.)

Kavungal Priya, E. P. Indira, Vadakkethil Balakrishnan Sreekumar and C. Renuka

Journal of Non-Timber Forest Products | Volume: 26 | Issue: 1 | Page No. 45-48 | 2019
DOI: https://doi.org/10.54207/bsmps2000-2021-565NN4 | Cite this article

Abstract

Calamus travancoricus Bedd. ex Becc. is a dioecious rattan species of commercial importance used in the furniture and handicraft industry. In order to develop genetic improvement and restoration programmes, it is essential to identify sex at seedling stage. In the present study, we have identified molecular markers linked to sex determination using RAPD markers. Of the twenty random primers standardised, the primer OPAU 02 yielded a unique amplicon specific to female genotypes. It is feasible to identify sex at the early stages of plant life, which is beneficial for improving further breeding programs in C. travancoricus.

Keywords

Calamus, Sex determination, DNA marker, RAPD markers, Dioecious rattan

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References

1. Ainsworth, C. (2000). Boys and girls come out to play: The molecular biology of dioecious plants. Annals of Botany. 86: 211- 221 https://doi.org/10.1006/anbo.2000.1201

Google Scholar

2. Al-Dous, E. K., George, B., Al-Mahmoud, M. E., Al-Jaber, M. Y., Wang, H., Salameh, Y.M., Al-Azwani, E. K., Chaluvadi, S., Pontaroli, A. C., DeBarry, J., Arondel, V., Ohlrogge, J., Saie, I. J., Suliman-Elmeer, K. M., Bennetzen, J. L., Kruegger, R. R. and Malek, J.A. (2011). De novo genome sequencing and comparative genomics of date palm (Phoenix dactylifera). Nature Biotechnology 29: 521-527 https://doi.org/10.1038/nbt.1860

Google Scholar

3. Alström-Rapaport, C., Lascoux, M., Wang, Y. C., Roberts, G. and Tuskan, G. A. (1998). Identification of RAPD marker linked to sex determination in the basket willow (Salix viminalis L.). Journal of Heredity 89: 44-49 https://doi.org/10.1093/jhered/89.1.44

Google Scholar

4. Dhawan, C., Kharb, P., Sharma, R., Uppal, S. and Aggarwal, R. A. (2013). Development of male-specific SCAR marker in date palm (Phoenix dactylifera L.). Tree Genetics Genomes 9: 1143-1150 https://doi.org/10.1007/s11295-013-0617-9

Google Scholar

5. Doyle, J. J. and Doyle, J.S. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11-15

Google Scholar

6. Gallagher, S. R. (1996). Quantification of DNA & RNA with absorption and fluorescence spectroscopy. In: Auubel, F. M., Brent, R., Kingsten, R.E., Moore, D.D., Siedman, J.G., Smith, J.A and   Struhl, K. (eds.), Current protocols in Molecular Biology. Vol III. pp A.3D.1 – A3D.8. John Wiley & Sons. USA

Google Scholar

7. Gangopadhyay, G., Roy, S. K., Ghose, K., Poddar, R., Bandyopadhyay, T., Basu, D. and Mukherjee, K. K. (2007). Sex detection of Carica papaya and Cycas circinalis in pre- flowering stage by ISSR and RAPD. Current Science 92: 524- 526

Google Scholar

8. George, J., Karun, A., Manimekalai, R., Rajesh, M. K. and Remya, P. (2007). Identification of RAPD markers linked to sex determination in palmyrah (Borassus flabellifer L.). Current Science 93: 1075-1077

Google Scholar

9. Hormaza, J. I., Dollo, L., Polito, V.S. (1994). Identification of RAPD marker linked to sex determination in Pistacia vera using bulked segregant analysis. Theoretical Applied Genetics 89: 9 - 13 https://doi.org/10.1007/BF00226975

Google Scholar

10. Jiang, C. X. and Sink, K. C. (1997). RAPD and SCAR markers linked to the sex expression locus M in Asparagus. Euphytica 3: 329-333 https://doi.org/10.1023/A:1002958007407

Google Scholar

11. Li. M., Yang H, Li F, Yang F, Yin G and Gan S. (2010). A malespecific SCAR marker in Calamus simplicifolius, a dioecious rattan species endemic to China. Mol Breed. 25:549-551 https://doi.org/10.1007/s11032-009-9349-9

Google Scholar

12. Li, T.S.C. and McLoughlin, C. (1997). Sea Buckthorn production guide. Canada Sea-buckthorn Enterprises Ltd. Availableonline, www.seabuckthorn.com

Google Scholar

13. Ng, C.Y., Wickneswari, R. and Choong, C.Y. (2014). Identification of floral genes for sex determination in Calamus palustris Griff. by using suppression subtractive hybridization. Genetics and Molecular Research 13: 6037 - 6049 https://doi.org/10.4238/2014.August.7.18

Google Scholar

14. Paran, I. and Michelmore, R. (1993). Development of reliable PCR based markers linked to Downy mildew resistance genes in lettuce. Theoretical Applied Genetics 85: 985- 993 https://doi.org/10.1007/BF00215038

Google Scholar

15. Parasnis, A. S., Gupta, V. S., Tamhankar, S. A. and Ranjekar, P. K. (2000): A highly reliable sex diagnostic PCR assay for mass screening of papaya seedlings. Molecular Breeding 6: 337-344 https://doi.org/10.1023/A:1009678807507

Google Scholar

16. Persson, H. A. and Nybom, H. (1998). Genetic sex determination and RAPD marker segregation in the dioecious species sea buckthorn (Hippophae rhamnoides L.). Hereditas 129: 45- 51 https://doi.org/10.1111/j.1601-5223.1998.00045.x

Google Scholar

17. Pickering, C. M. and Hill, W. (2002). Reproductive ecology and the effect of altitude on sex ratios in the dioecious herb Aciphylla simplicifolia (Apiaceae). Australian Journal of Botany 50: 289-300 https://doi.org/10.1071/BT01043

Google Scholar

18. Rana, S., Shirkot, P. and Yadav, M. C. (2009). A female sex associated Randomly amplified polymorphic DNA marker in dioecious Hippophae salicifolia. Genes, Genomes and Genomics 3 (1): 96-101

Google Scholar

19. Renner, S. S. and Ricklefs, R. E. (1995). Dioecy and its correlates in the flowering plants. American Journal of Botany 82: 596-606 https://doi.org/10.1002/j.1537-2197.1995.tb11504.x

Google Scholar

20. Sanghamitra, S., Geetha, K. A., Hidayath, K. P. and Maiti, S. (2010). Identification of RAPD markers linked to sex determination in guggal [Commiphora wightii (Arnott.)] Bhandari. Plant Biotechnology Reports 4 (1): 95-99 https://doi.org/10.1007/s11816-009-0113-8

Google Scholar

21. Sarmah, P. and Sarma. R. N. (2011). "Identification of a DNA marker linked to sex determination in Calamus tenuis Roxb., an economically important rattan species in northeast India." Molecular Breeding 27 (1): 115-118 https://doi.org/10.1007/s11032-010-9510-5

Google Scholar

22. Sharma, A., Zinta, G., Rana, S. and Shirkot, P. (2010). Molecular identification of sex in Hippophae rhamnoides L. using isozyme and RAPD markers. Forestry Studies in China 12(2): 62-66 https://doi.org/10.1007/s11632-010-0012-7

Google Scholar

23. Shibu, M. P., Ravishankar, K. V., Anand, L., Ganeshaiah, K. N. and Shaanker, U. (2000). Identification of sex-specific DNA markers in the dioecious tree, nutmeg (Myristica fragrans Houtt.). Plant Genetics Resource Newsletter 121: 59-61

Google Scholar

24. Singh, M., Kumar, S., Singh, A. K. and Kalloo, G. (2002). Female sex associated marker in pointed ground (Trichosanthes dioica Roxb.). Current Science 82: 131-132

Google Scholar

25. Sreekumar, V.B., Renuka, C., Suma, T.B. and Balasundaran, M. (2006). Taxonomic reconsideration of Calamus rivalis Thw. Ex Trim. and C. metzianus Schlecht (Arecaceae) through morphometric and molecular analyses. Botanical Studies 47: 443-452

Google Scholar

26. Thatte, K. S. and Deodhar, M.A. (2012). Study of flowering behaviour and sex determination in Gracinia indica (Thomas-Du Pettite) Choisy by means of molecular markers. Biotechnology 11 (4): 232-237 https://doi.org/10.3923/biotech.2012.232.237

Google Scholar

27. Williams, J. G. K., Kubelik, A.R., Livak, K. J., Rafalski J.A. and Tingey, S.V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 6231 - 6235 https://doi.org/10.1093/nar/18.22.6531

Google Scholar

28. Yang, H., Si-Ming, G., Huang-Tian, Y. and Huang-Can, X. (2005). Identification of random amplified polymorphic DNA markers linked to sex determination in Calamus simplicifolius. Journal of Integrative Plant Biology 47: 1249-1253 https://doi.org/10.1111/j.1744-7909.2005.00113.x

Google Scholar

29. Younis, R. A. A., Ismail, O. M. and  Soliman, S. S. (2008). Identification of sex- specific DNA markers for date palm (Phoenix dactylifera L.) using RAPD and ISSR techniques. Research Journal Agriculture and Biological Science  4(4): 278 - 284

Google Scholar

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How to cite

Priya, K., Indira, E.P., Sreekumar, V.B. and Renuka, C., 2019. Identification of female sex-specific DNA marker in a dioecious rattan (Calamus travancoricus Bedd. ex Becc.). Journal of Non-Timber Forest Products, 26(1), pp.45-48. https://doi.org/10.54207/bsmps2000-2021-565NN4

Publication History

Manuscript Published on 24 September 2021

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