Research Article | Published: 01 December 2010

In vitro propagation of Dendrocalamus hamiltonii Nees & Arn. ex Munro through nodal explants from mature culms

Prabha Bisht, Manu Pant, Abhinav Kant and Rayees Afzal Mir

Journal of Non-Timber Forest Products | Volume: 17 | Issue: 4 | Page No. 401-408 | 2010
DOI: https://doi.org/10.54207/bsmps2000-2010-6H3191 | Cite this article

Abstract

An efficient procedure for in vitro propagation through axillary bud proliferation has been developed for Dendrocalamus hamiltonii Nees & Arn. ex Munro. Best results for bud induction were obtained in liquid MS medium supplemented with 15µM BAP. MS supplemented with 10µM BAP was found to be optimal for shoot multiplication giving an average shoot multiplication rate of 9.13 folds. Multiplied shoots were further cultured onto MS medium supplemented with auxin for in vitro rooting. Optimal rooting (66.13%) was obtained on MS medium supplemented with 25µM IBA in four weeks. The in vitro raised plantlets with well developed shoots and roots were subsequently hardened in green house conditions. A survival rate of over 90% was achieved on transferring the plants to field conditions where they exhibited normal growth.

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How to cite

Bisht, P., Pant, M., Kant, A. and Mir, R.A., 2010. In vitro propagation of Dendrocalamus hamiltonii Nees & Arn. ex Munro through nodal explants from mature culms. Journal of Non-Timber Forest Products, 17(4), pp.401-408. https://doi.org/10.54207/bsmps2000-2010-6H3191

Publication History

Manuscript Published on 01 December 2010

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