Research Article | Published: 01 September 2016

In vitro multiplication of Coleus barbatus (Patharchur) in response to various growth regulatory hormones

Meena Bakshi, Aanchal Mittal and Chanderkant Tewari

Journal of Non-Timber Forest Products | Volume: 23 | Issue: 3 | Page No. 127-130 | 2016
DOI: https://doi.org/10.54207/bsmps2000-2016-8784V3 | Cite this article

Abstract

Coleus barbatus (Patharchur) - an important medicinal species was multiplied through in vitro techniques in response to various growth regulatory hormones. Sterilization of explants was carried out with 0.1% Mercuric chloride for 10 min. Inoculation of explants on MS (Murashige and Skoog, 1962) medium supplemented with lower concentration of auxin NAA (0.25mg/L) and higher concentration of cytokinin BAP (1mg/L) proved quite effective. The maximum rooting % (100%) and maximum number of roots (8) were found in plants cultured on ½ strength MS media supplemented with NAA (1mg/L) while the length of roots was maximum with IBA (1mg/L). The plantlets were hardened, acclimatized and successfully transferred to the field conditions which showed 96% survival.

Keywords

Coleus barbatus , Sterilization, Hormones, Micropropagation, Acclimatization

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How to cite

Bakshi, M., Mittal, A. and Tewari, C., 2016. In vitro multiplication of Coleus barbatus (Patharchur) in response to various growth regulatory hormones. Journal of Non-Timber Forest Products, 23(3), pp.127-130. https://doi.org/10.54207/bsmps2000-2016-8784V3

Publication History

Manuscript Published on 01 September 2016

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