Research Article | Published: 01 March 2010

Molecular and chemical variability studies in Dioscorea Oppositifolia L. in Tirunelveli Hills in South India

A. John De Britto and N. Nirmal Kumar

Journal of Non-Timber Forest Products | Volume: 17 | Issue: 1 | Page No. 87-91 | 2010
DOI: https://doi.org/10.54207/bsmps2000-2010-8D655C | Cite this article

Abstract

In the present study, Dioscorea oppositifolia L was collected from ten locations in Tirunelveli hills and the genetic variability was investigated using RAPD - PCR fingerprint and the populations which showed high percentage of polymorphism was selected. Those populations were chosen for further phytochemical analysis. The active principle in these plants has been quantified by HPLC analysis. The population which exhibited both high percentage of polymorphism and high amount of active principle was considered to be the superior genotype.

Keywords

Access Options

250/-

Buy Full Access in HTML Format

Instant access to the full article.

Get access to the full version of this article. Buy Full Access in HTML Format

References

1. Allendorf F.W and Leary R.F. (1986). Heterozygosity and fitness in natural populations of animals in Soule. Conservation Biology, 57-76.

Google Scholar

2. Doyle J.J and Doyle J.L. (1987). Rapid DNA isolation procedures for small quantities of fresh leaf tissues. Phytochemistry, 19:11-15.

Google Scholar

3. Hamrick J.L and Godt M.J.W. (1996). Conservation genetics of endemic plant species. Cryoletters, 17-22.  https://doi.org/10.1007/978-1-4757-2504-9_9

Google Scholar

4. Nei N.S. and Li W. (1979). Mathematical model for studying Genetic variation in terms of Restriction Endonucleases. Proc. Natl. Acad. Sci., 76: 5269-5273.  https://doi.org/10.1073/pnas.76.10.5269

Google Scholar

5. Nei. (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89:583.  https://doi.org/10.1093/genetics/89.3.583

Google Scholar

6. Sneath D.H.A and Sokal R. (1973). Numerical Taxonomy. Freedom Publishers Pvt. Ltd. 27-38.

7. Tikhomiroff C and Jolicoeur. (2002). Screening of Catharanthus roseus secondary metabolites by high performance liquid chromatography. J. Chromato., 955:87-93.  https://doi.org/10.1016/S0021-9673(02)00204-2

Google Scholar

8. Williams J.G.K., Kubelik A.R., Livak. K.J., Rfalski. J.A. and Tingey. S.V. (1990). DNA polymorphisms amplified by arbitrary primers used as genetic markers.  Nucleic acid Research, 18:6531-6535.  https://doi.org/10.1093/nar/18.22.6531

Google Scholar

9. Wilson E.O. (1992). The Diversity of Life. Silver Holten Publishers Pvt. Ltd. Penguin. 17-25.

About this article

How to cite

De Britto, A.J. and Kumar, N.N., 2010. Molecular and chemical variability studies in Dioscorea Oppositifolia L. in Tirunelveli Hills in South India. Journal of Non-Timber Forest Products, 17(1), pp.87-91. https://doi.org/10.54207/bsmps2000-2010-8D655C

Publication History

Manuscript Published on 01 March 2010

Share this article

Anyone you share the following link with will be able to read this content: