Research Article | Published: 01 December 2010

Standardization of genomic DNA extractions from lac-host trees, Schleichera oleosa and Butea monosperma, for PCR-based genetic diversity studies

Dipnarayan Saha

Journal of Non-Timber Forest Products | Volume: 17 | Issue: 4 | Page No. 395-400 | 2010
DOI: https://doi.org/10.54207/bsmps2000-2010-9S8280 | Cite this article

Abstract

Isolation of good quality genomic DNA from the polysaccharide and phenol-rich leaf tissues were attempted from Kusum (Schleichera oleosa) and Palas (Butea monosperma) trees, to be used in PCR-based genetic diversity studies. Out of three different methods tried, a CTAB-based method adapted from Doyle and Doyle (1990) was found suitable for isolation of good quality genomic DNA from the young leaves of Kusum and Palas trees. The genomic DNA isolated from young leaves of Kusum and Palas through above method were found to have satisfactory purity index (>1.7) at 260/280 nm absorption and high yield as well. The genomic DNA isolated through CTAB method from the young leaves of Kusum and Palas were found suitable for RAPD-PCR reactions.

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How to cite

Saha, D., 2010. Standardization of genomic DNA extractions from lac-host trees, Schleichera oleosa and Butea monosperma, for PCR-based genetic diversity studies. Journal of Non-Timber Forest Products, 17(4), pp.395-400. https://doi.org/10.54207/bsmps2000-2010-9S8280

Publication History

Manuscript Published on 01 December 2010

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