Research Article | Published: 01 June 2015

Improved in vitro propagation of Rose cultivars against varying concentrations of carbon source, agar and plant growth regulators

Kumud Saklani, Hem Pant, Vinod Bisht, Arun Kumar Singh and Vijay Rawat

Journal of Non-Timber Forest Products | Volume: 22 | Issue: 2 | Page No. 69-73 | 2015
DOI: https://doi.org/10.54207/bsmps2000-2015-B51YYC | Cite this article

Abstract

The present study was conducted to improve upon the micropropagation protocol of Rose cultivars by modification of the MS medium through variations in sucrose and agar concentrations thereby enhancing the shoot multiplication and rooting efficiency of the nodal explants. High sucrose concentrations and low agar concentrations favoured shoot mutiplication during the in vitro stages. Enhanced multiplcation and growth was observed on sub culturing the mother explants with regenerated shoots on fresh MS medium containing sucrose (3.5%, w/v) and agar (0.6%, w/v), supplemented with lower concentrations cytokinin combination of BAP and KN (2.5 mgl-1+1.5 mgl-1) respectively. Half strength MS medium containing sucrose (2.0%, w/v) and agar (0.3%, w/v) with NAA and BAP (2.0 mgl-1+0.5 mgl-1) in combination was most effective for rooting.

Keywords

Rose Cultivars, MS Medium, Sucrose, Agar, Plant Growth Regulators, Shoot Multiplication and Rooting

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How to cite

Saklani, K., Pant, H., Bisht, V., Singh, A.K. and Rawat, V., 2015. Improved in vitro propagation of Rose cultivars against varying concentrations of carbon source, agar and plant growth regulators. Journal of Non-Timber Forest Products, 22(2), pp.69-73. https://doi.org/10.54207/bsmps2000-2015-B51YYC

Publication History

Manuscript Published on 01 June 2015

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