Invited Commentary | Published: 31 December 2020

Biochemical and Molecular Characterization of Bamboo Variants

Bhaskar Jyoti Dey, Ashok Kumar Verma, Salil Kumar Tewari and Ashutosh Dubey

Journal of Non-Timber Forest Products | Volume: 27 | Issue: 1 | Page No. 50-55 | 2020
DOI: https://doi.org/10.54207/bsmps2000-2020-HQNN97 | Cite this article

Abstract

Classical taxonomic studies of the bamboos are based on floral morphology and growth habit, which can cause problems in identification due to erratic flowering. Identification and genetic relationships in 18 species of bamboo were investigated using SDS-PAGE and RAPD analysis. Protein profiles of 18 bamboo species revealed clear polymorphism which can be used to identify species. Dendrogram based on SDS-PAGE data separated 18 species into two major clusters with Bambusa nutans & B. polymorpha, Gigantochloa andamanica & Dendrocalamus longispathus, G. andamanica & D. gigangteus showed maximum similarity of 71.4% in each case while genotype B. tulda and B. nutans (10.4%) showed lowest similarity. On the other hand, RAPD analysis proved to be a better tool with 14.67 as an average number of bands per polymorphic primers. Dendrogram based on RAPD separated 18 species into 2 major clusters with D. membranaceous and B.multiplex showed maximum similarity of 83% while genotype D. asper and B. balcooa showed least similarity (10.6%).

Keywords

Cluster analysis, Polymorphism, Primer, Protein profiles, RAPD, SDS-PAGE, Total soluble proteins

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How to cite

Dey, B.J., Verma, A.K., Tewari, S.K. and Dubey, A., 2020. Biochemical and Molecular Characterization of Bamboo Variants. Journal of Non-Timber Forest Products, 27(1), pp.50-55. https://doi.org/10.54207/bsmps2000-2020-HQNN97

Publication History

Manuscript Received on 29 April 2020

Manuscript Revised on 08 August 2020

Manuscript Accepted on 14 August 2020

Manuscript Published on 31 December 2020

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