Research Article | Published: 01 March 2009

In vitro multiplication of Jojoba (Simmondsia chinensis L.)

V. Dalal, R. S. Dhillon and M. Jattan

Journal of Non-Timber Forest Products | Volume: 16 | Issue: 1 | Page No. 45-49 | 2009
DOI: https://doi.org/10.54207/bsmps2000-2009-OT41DT | Cite this article

Abstract

A protocol for micro propagation for known sexuality of Jojoba was developed and standardized. Nodal explants from actively growing young shoots were used to proliferate in MS medium supplemented with different concentrations of cytokinins and auxins. Pre-treatment with bavistin (0.2% for 3 min.) followed by HgCl2 (0.1% for 5 min.) was found to be the best for disinfecting the explants. Maximum proliferation in nodal explants was recorded on MS medium containing 2.0 mg l-1 BAP. The medium having MS + 0.5 mg 1-1 BAP + 0.1 mg 1-1 IAA produced maximum number of shoots (5.7) per explant. Elongation of in vitro multiplied shoots was found maximum in MS + 2.0 mg 1-1 BAP. The highest rooting (63.7 percent) was observed in full strength MS medium supplemented with 0.5 mg 1-1 IBA. The in vitro raised plants were hardened for survival after pricking out in different potting media kept in a mist chamber with gradually decreasing humidity regime. Sand, soil and vermicompost mixture in ratio of 3:1:1 was found suitable and recorded 83 percent survival of in vitro raised clonal plants.

Keywords

Access Options

250/-

Buy Full Access in HTML Format

Instant access to the full article.

Get access to the full version of this article. Buy Full Access in HTML Format

References

1. Adams, J. A., Johnson, H. B., Bingham, F. T. and Yermonas, D. M. (1977). Gaseous exchange of Simmondsia chinensis (Jojoba) measured with a double isotope porometer and related to water stress, salt stress and nitrogen deficiency. Crop Science, 17: 11-15.  https://doi.org/10.2135/cropsci1977.0011183X001700010004x

Google Scholar

2. Benzioni, A. and Forti, M. (1989). Jojoba. In: Oil Crops of the World (G Robbelen; R.K. Downey and A. Ashu, Eds.). pp. 448-461. McGraw-Hill Publishing Co.1, New York.

3. Bhatia, V. K. (2001). Potential of Jojoba oil non-energy applications. In: Proceedings of National Seminar on Production, Marketing and Processing of Jojoba (Simmondsia chinensis), held at Jaipur (Raj.), India, February 19-20, 2001. pp.17-21.

4. Boven, M. V., Daenens, P., Kass, K and Kotelaire, K. (1997). Content and composition of free sterols and free fatty alcohols in Jojoba oil. J. Agric Food Chem., 45: 1180-1184.  https://doi.org/10.1021/jf960488g

Google Scholar

5. Frankel, A. (1976). Industrial use of whale oil. Chem. Tech., 6: 396-398.

Google Scholar

6. Gentry, H. S. (1958). The natural history of Jojoba (Simmondsia chinensis L.) and its cultural aspects. Econ. Bot., 12: 261-295.  https://doi.org/10.1007/BF02859772

Google Scholar

7. Harsh, L.N., Tewari, J.C., Bohra, M.D. and Tripathi, D. (2001). Standardization of agronomic practices of Jojoba cultivation in arid regions. In: Pro. of National Seminar on "Production, Marketing and Processing of Jojoba (Simmondsia chinensis)", held at Jaipur (Raj.) India, from February 19-20, 2001. pp. 41-46.

Google Scholar

8. Khanam, A., Rao, Y.B.N. and Farook, S.A. (1999). Standard in vitro experimental protocol for high frequency mass micropropagation of Jojoba (Simmondsia chinensis L. Schneider). Advances in Plant Sciences, 12: 361-366.

Google Scholar

9. Murashige, T. and Skoog. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant, 15: 475-497.  https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Google Scholar

10. Nandwani, D., Sharma, R. and Ramawat, K.G. (1996). High frequency regeneration in callus cultures of a tree, Tecomella undulata. Gartenbanwissenschaft, 61: 147-150.

Google Scholar

11. N.R.C. (1985). Jojoba: New Crop for Arid Lands, New Raw Material for Industry. National Research Council National Academy Press, Washington, D.C.

Google Scholar

12. Rathore, J.S., Singh, R.P. and Shekhawat, N.S. (1991). Clonal propagation of desert Teak (Tecomella undulata) through tissue culture. Plant Science, 79: 217-222.  https://doi.org/10.1016/0168-9452(91)90108-K

Google Scholar

13. Roussos, P.A., Tolia, M.A., Pontikis, C.A. and Kotsias, D. (1999). Rapid multiplication of Jojoba seedlings by in vitro culture. Plant Cell, Tissue and Organ Culture, 57: 133-137. https://doi.org/10.1023/A:1006316732621

Google Scholar

14. Sardana, J., Batra, A. and Ali, D.J. (2000). An expeditious method for regeneration of somatic embryos in Jatropha curcas L. Phytomorphology, 50: 239-242.

Google Scholar

15. Yermonas, D. M. (1979). Jojoba- A crop whose time has come. California Agriculture, 33: 4-11.

Google Scholar

16. Yermonas, D. M. and Duncan, C. C. (1976). Quantitative and qualitative characteristics on Jojoba seed. J. Am. Chem. Soc., 53: 80-87.  https://doi.org/10.1007/BF02637398

Google Scholar

About this article

How to cite

Dalal, V., Dhillon, R.S. and Jattan, M., 2009. In vitro multiplication of Jojoba (Simmondsia chinensis L.). Journal of Non-Timber Forest Products, 16(1), pp.45-49. https://doi.org/10.54207/bsmps2000-2009-OT41DT

Publication History

Manuscript Published on 01 March 2009

Share this article

Anyone you share the following link with will be able to read this content: