Research Article | Published: 25 March 2018

Effect of Chilling Treatment and Different Growth Media on Seed Germination and Seedling Growth Performance of Picrorhiza kurrooa: An Endangered Medicinal Herb

Dharam Chand Attri, Mohan Chandra Nautiyal, Vijay Laxmi Trivedi and Jyoti  Sati

Journal of Non-Timber Forest Products | Volume: 25 | Issue: 1 | Page No. 57-62 | 2018
DOI: https://doi.org/10.54207/bsmps2000-2018-LU4UF8 | Cite this article

Abstract

An experimental trial was conducted to access the impact of cold stratification and different growth media on seed germination and seedling growth performance of Picrorhiza kurrooa under ex-situ conditions at lower altitude. The experiment was carried out in CRD (Completely Randomized Design) with 20 treatments, three replications and 60 seeds per treatment. Influence of growth media on seed germination was measured by thirteen parameters. Parameters such as minimum and maximum days required for initial (8.00±1.00) and final (28.67±2.08) germination was observed in T10 (Soil+Sand+Azotobacter+Cocopeat) and T18 (Soil+FYM+Cocopeat+Rockphosphate+Vermicomposte). The maximum germination percentage (83.33±8.82%), plant height (2.50±0.10) and number of shoots (3.67±0.58) were recorded in T5 (Soil+Sand+FYM+Moss). The maximum number of leaves (9.00±3.46), leaf length (1.53±0.35), leaf width (0.93±0.15), shoot length (2.70±0.26), number of roots (4.33±2.31) and root length (2.63±0.15) was found significantly superior  in T18 (Soil+FYM+Cocopeat+Rockphosphate +Vermicomposte).

Keywords

Chilling Stratification, Growth Media, Picrorhiza kurrooa, Seeds, Endangered

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References

1. Banyal, H.S., Rani and Devi, N. (2014) "Picrorhiza kurroa Royal Ex Benth exhibits antimalarial activity against Plasmodium berghei vincke and lips, 1948", Asian Journal of Biological Sciences https://doi.org/10.3923/ajbs.2014.72.75

Google Scholar

2. Baskin, C.C. and Baskin, J.M. (2014). Seeds: ecology, biogeography, and evolution of dormancy and germination, 2nd edn. Academic Press, London

Google Scholar

3. Bhardwaj, R.L. (2014). Effect of growing media on seed germination and seedling growth of Papaya cv. 'Red Lady'. African Journal of Plant Science, 8(4): 283-28 https://doi.org/10.5897/AJPS11.265

Google Scholar

4. Billings, W.D. and Mooney, H.A. (1968). The ecology of arctic and alpine plants. Biological Reviews 43: 481-529 https://doi.org/10.1111/j.1469-185X.1968.tb00968.x

Google Scholar

5. Bonomi, C., Rossi, G., Mondoni, A. (2005). Seed conservation activities for narrow endemic species in the italian Alps. XVII International Botanical Congress. Vienna, Austria

Google Scholar

6. Chaturvedi, G.N. and Singh, R.H. (1966). Jaundice of infectious hepatitis and its treatment with an indigenous drug, Picrorhiza kurrooa. J Res Ind Med 1:1-13

Google Scholar

7. Chettri, N., Sharma, E. And Lama, S.D. (2005). Non-timber forest produces utilization, distribution and status in a trekking corridor of Sikkim, India, Lyonia; 8: 89-101

Google Scholar

8. Ferrarini, A., Rossi, G., Parolo, G. and Ferloni, M. (2008). Planning low-impact tourist paths within a Site of Community Importance through the optimisation of biological and logistic criteria. Biological Conservation 141: 1067-1077 https://doi.org/10.1016/j.biocon.2008.01.013

Google Scholar

9. Forbis, T.A. (2003) Seedling demography in an alpine ecosystem. Amer. J. Bot. 90:1197 https://doi.org/10.3732/ajb.90.8.1197

Google Scholar

10. Korner, C. (2003). Alpine plant life: functional plant ecology of high mountain ecosystems, 2nd edn. Springer-Verlag, Berlin

Google Scholar

11. Malaisamy, A. and Ravindran, C. (2003). Medicinal Plants: Where we do stand globally? Science Tech Entrepreneur Magazine 2(5): 42-49

Google Scholar

12. Mallick, M.N., Singh, M., Parveen, R., Khan, W., Ahmad, S., Najm, M.Z. and Husain, S.A, (2015) HPTLC analysis of bioactivity guided anticancer enriched fraction of hydroalcoholic extract of Picrorhiza kurroa, BioMed Research International https://doi.org/10.1155/2015/513875

Google Scholar

13. Malviya, N., Jain S. and Malviya, S. (2011) Antiasthmatic potential of Indigenous medicinal plants, Journal on Complementary Medicine and Drug Discovery, vol. 1(1), 43-50, 2011 https://doi.org/10.5455/spatula.20101223032951

Google Scholar

14. Mohammad, N., Dahayat, A. and Mishra, Y. (2016). Effect of seed treatments and potting medium on seed germination and associate parameters in critically endangered Litsea glutinosa (Lour.) Indian Journal of Forestry 39 (2): 111-115

Google Scholar

15. Nautiyal, B.P., Parkash, V., Chauhan, R.S., Purohit, H. and Nautiyal, M.C. (2001). Assessment of germinability, productivity and cost benefit analysis of Picrorhiza kurrooa cultivated at lower altitude. Current Science 81(5): 579-585

Google Scholar

16. Nautiyal, M.C. and Nautiyal, B.P. (2004). Agrotechniques for High Altitude Medicinal and Aromatic plants. Silver jubille Publication of HAPPRC. Bishen Singh Mahendra Pal singh, Dehradun

Google Scholar

17. Nayar, M.P. and Sastri, A.R.K. (1990). Red data plants of India. CSIR Publication, New Delhi

Google Scholar

18. Ozenda, P. (1985) La végétation de la chaîne alpine dans l'espace montagnard européen. Masson, Paris

Google Scholar

19. Parolo, G. and Rossi, G. (2008). Upward migration of vascular plants following a climate warming trend in the Alps. Basic and Applied Ecology 9: 100-107 https://doi.org/10.1016/j.baae.2007.01.005

Google Scholar

20. Purohit, H.C., Nautiyal, B.P. and Nautiyal, M.C. (2008). Interpopulation Varition in P. kurrooa Royle ex. Benth-Step towards Identifying Genetic Variability and Elite Strains for Crop Improvement Study. American J. of Plant Physiol. 3: 154-164 https://doi.org/10.3923/ajpp.2008.154.164

21. Rai, L.K., Prasad, P. and Sharma, E. (2000). Conservation threats to some important medicinal plants of the Sikkim Himalaya. Bio.Conserv., 93:27-53 https://doi.org/10.1016/S0006-3207(99)00116-0

Google Scholar

22. Rossi, G., Parolo, G. and Aplin, D. (2007). Ex situ conservation reaches out to save upwardly-mobile plants from extinction. European Native Seed Conservation Newsletter 3: 3-4

Google Scholar

23. Rossi, G., Parolo, G. and Ferrarini, A. (2008). A rapid and cost-effective tool for managing habitats of the European Natura 2000 network: a case study in the Italian Alps. Biodiversity and Conservation 18: 1375-1388 https://doi.org/10.1007/s10531-008-9459-4

Google Scholar

24. Sah, J.N. and Varshney, V.K. (2013). Chemical constituents of Picrorhiza genus: a review. Am J Essent Oils Nat Prod 1:22-37

Google Scholar

25. Sane, S.A., Shakya, N. and Gupta, S. (2011). Immunomodulatory effect of picroliv on the efficacy of paromomycin and miltefosine in combination in experimental visceral leishmaniasis, Experimental Parasitology, 127 : 376-381 https://doi.org/10.1016/j.exppara.2010.09.003

Google Scholar

26. Sanjay, S., Banu, S.H. and Chetankumar, M. (2015). "The study of potentiality of Picrorhiza kurroa root proteins to inhibit free radicals and α-amylase enzyme", Asian Journal of pharmaceutical and clinical research, vol. 8(2): 220-225

Google Scholar

27. Shimono Y, Kudo G (2005) Comparisons of germination traits of alpine plants between fellfield and snowbed habitats. Ecol. Res. 20:189-197 https://doi.org/10.1007/s11284-004-0031-8

Google Scholar

28. Shitiz. K., Pandit, S., Chauhan, R.S. and Sood, H. (2013). Picrosides content in the rhizomes of Picrorhiza kurrooa Royle ex. Benth. traded for herbal drugs in the markets of North India. Int. J. Med. Aromat Plants 3:226-233 Delhi

Google Scholar

29. Thuiller, W. (2007). Climate change and the ecologist. Nature 448: 550-552 https://doi.org/10.1038/448550a

Google Scholar

30. Tiwari, S.S., Pandey, M.M., Srivastava, S. and Rawat, A.K. (2012) TLC densitometric quantification of picrosides (Picroside-I and Picroside-II) in Picrorhiza kurroa and its substitute Picrorhiza scrophulariiflora and their antioxidant studies, Biomedical Chromatography: BMC, 26 (1) : 61-8 https://doi.org/10.1002/bmc.1626

Google Scholar

31. UNEP.(1992). Convention on biological diversity. United Nations Environmental Programme

Google Scholar

32. Uniyal, A., Uniyal, S.K. and Rawat, G.S. (2011). Commercial Extraction of Picrorhiza kurrooa Royle ex Benth. in the Western Himalaya. Mountain Research and Development, 31(3): 201-208 https://doi.org/10.1659/MRD-JOURNAL-D-10-00125.1

Google Scholar

33. Ved, D.K. and Goraya, G.S. (2008). Demand and supply of Medicinal Plants in India. National Medicinal Plant Board, New Delhi and M/s Bishen Singh Mahendra Pal Singh, Dehradun

Google Scholar

34. Wenk, E.H. and Dawson, T.E. (2007). Interspecific differences in seed germination, establishment, and early growth in relation to preferred soil type in an alpine community. Arct. Antarct. Alp. Res. https://doi.org/10.1657/1523-0430(2007)39[165:IDISGE]2.0.CO;2

Google Scholar

35. Yegnarayan, R., Dange, S.V., Vaidya, S.D., and Balwani, M. (1982). Study of Picrorhiza kurrooa (PK300) in case of bronchial asthma. Bombay Hosp J 24:81-84

Google Scholar

36. Zhang, D.K., Yu, J.J., Li, Y.M., Wei, L.N., Yu, Y., Feng, Y.H. and Wang, X. (2012) A Picrorhiza kurroa derivative, picroliv, attenuates the development of dextran-sulfate-sodium-induced colitis in mice, Mediators of Inflammation, 2012 :751629 https://doi.org/10.1155/2012/751629

Google Scholar

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How to cite

Attri, D.C., Nautiyal, M.C., Trivedi, V.L. and Sati, J., 2018. Effect of Chilling Treatment and Different Growth Media on Seed Germination and Seedling Growth Performance of Picrorhiza kurrooa: An Endangered Medicinal Herb. Journal of Non-Timber Forest Products, 25(1), pp.57-62. https://doi.org/10.54207/bsmps2000-2018-LU4UF8

Publication History

Manuscript Published on 25 March 2018

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