Research Article | Published: 01 June 2002

In-vitro propagation of bixa orellana - A natural dye producing tree

B. Muniswamy and H. L. Sreenath

Journal of Non-Timber Forest Products | Volume: 9 | Issue: 1/2 | Page No. 1-4 | 2002
DOI: https://doi.org/10.54207/bsmps2000-2002-T3WRL5 | Cite this article

Abstract

Direct somatic embryogenesis and plant regeneration was achieved from excised immature zygotic embryos of Bixa orellana - a ornamental and natural dye producing tree. The zygotic embryos were cultured initially on half-strength MS (Murashige and Skoog’s, 1962) medium supplemented with 0.1 mgl-1 of Kn. Though, germination of immature zygotic embryos and plants regeneration was achieved in all the three media compositions tested. However, in one medium containing Kn (0.1 mgl-1), about 10 per cent direct somatic embryos were produced on the hypocotyl portions. After culturing for 30 days, high frequency of somatic embryogenesis and maturation of somatic embryos was obtained in the same medium. These somatic embryos could be germinated into plantlets by giving 2-3 sub-cultures at monthly intervals onto half-strength MS medium supplemented with 0.1 mgl-1 of Kn. The regenerated plants having 2-3 cm long shoots and 2-3 pairs of leaves with roots were hardened in polybags containing soil mixture and subsequently established in field.

Keywords

Access Options

250/-

Buy Full Access in HTML Format

Instant access to the full article.

Get access to the full version of this article. Buy Full Access in HTML Format

References

1. Anonymous (1956). Dictionary of Gardening - a practical and scientific encyclopedia of Horticulture. Vol. I, pp. 284-285.

Google Scholar

2. Anonymous (1960). Encyclopedia of Textiles. Doric Publishing Company, America, p. 471.

3. Bailey, L.H. (1964). Mannual of Cultivated Plants. The Macmillan Company, New York, p. 682.

4. Gupta, D. (1992). Natural Dyes and Their Application to Textiles. In: M.L. Gulrajani and D. Gupta (eds.) Dept. of Textile Technology. pp. 32-33. Indian Institute of Technology, New Delhi.

Google Scholar

5. Dublin, P. (1981). Embryogenese somatique directe sur fragments de feceilles de cafeier Arabusta. Café Cocao The., 25: 237-241.

Google Scholar

6. Hu, C. and Wang, P. (1986). Embryo culture: Techniques and Applications. In: Hand Book of Plant Cell Culture. Vol. 4, D.A. Evans, W.R. Sharp and P.V. Ammirato (eds.), pp. 43-94. Mac Millan Publishing Co., New York.

Google Scholar

7. Jayashree, G.; Samuel Genesh, D. and Sreenath, H.L. (1995). Effect of Kinetin on germination of somatic embryos in C x R cultivar of coffee. J. Coffee Res., 25(2): 102-105.

Google Scholar

8. Madhuri, M. and D’Souza, M.C. (2000). In vitro clonal propagation of Annotto (Bixa orellana). Cur. Sci., 78: 1532-1535.

Google Scholar

9. Morel, G. (1964). Rev. Cytol. Cytophysiol Veget. 27: 307-314.

10. Muniswamy, B. and Sreenath, H.L. (1995). Genotypes specificity of direct somatic embryogenesis from cultured zygotic embryos in coffee. In: Recent Advances on Biotechnological Applications of Plant tissue and Cell culture. G.A. Ravishanker and L.V. Venkataraman (eds.), pp. 204-209. Oxford and IBA Publishing Co. Pvt., New Delhi.

11. Muniswamy, B. and Sreenath, H.L. (1998). In vitro plant development from cultured zygotic embryos of C. bengalensis. In: Developments in Plantation Crops Research, N.M. Mathew and C. Kuruvilla Jacob (eds.), pp. 83-85.

12. Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant. 15: 473-497.  https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Google Scholar

About this article

How to cite

Muniswamy, B. and Sreenath, H.L., 2002. In-vitro propagation of bixa orellana - A natural dye producing tree. Journal of Non-Timber Forest Products, 9(1/2), pp.1-4. https://doi.org/10.54207/bsmps2000-2002-T3WRL5

Publication History

Manuscript Published on 01 June 2002

Share this article

Anyone you share the following link with will be able to read this content: