Research Article | Published: 01 December 2003

In vitro multiplication of Strychnos potatorum Linn.f. from root explants - A medicinally important plant

M. Praveen, A. Lakshman, T. Yugandhar and N. Ramaswamy

Journal of Non-Timber Forest Products | Volume: 10 | Issue: 3/4 | Page No. 230-234 | 2003
DOI: https://doi.org/10.54207/bsmps2000-2003-X76JBE | Cite this article

Abstract

Complete plantlets were regenerated in vitro from root explants of Strychnos potatorum Linn.f. (Chilla) on Murashige and Skoog’s (MS) medium supplemented with various concentrations of cytokinins.  Multiple shoot induction occurred in root explants on MS medium supplemented with 1-4 mgl-1 benzylaminopurine or Kinetin. Maximum numbers of shoots/explant (18.4 + 0.2) were induced on MS medium fortified with 2 mgl-1 BAP. Shoots obtained from BAP and Kn amended media developed roots in 100% cultures on half strength MS basal medium fortified with Indole butyric acid (1 mgl-1).  Hardening and establishment of regenerated plantlets was achieved in green house.

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References

1. Anonymous (1956). The Wealth of India, Raw Materials, Vol. X (pp. 66-67). Publication and Information Directorate, CSIR, New Delhi.

Google Scholar

2. Gharyal, P.K. and Maheshwari, S.C. (1983). In vitro differentiation of plantlets from tissue culture of Albizia lebbeck L. Plant Cell Tiss. and Org. Cult., 2: 49-53.  https://doi.org/10.1007/BF00033552

Google Scholar

3. Greaves, A. (1982). Pinus oocarpa. Forestry Abst., 43: 503-532.

Google Scholar

4. Hattori, M.; Nakabayashi, T.; Lim, Y.A.; Miyashiro, H.; Kurokawa, M.; Shiraki, K.; Gupta, M.P.; Correa, M. and Pilapitiya, V. (1995).  Inhibitory effect of various ayurvedic and panamanion medicinal plants on the infection of herpes simplex – virus 1. In vitro and in vivo. Phytotherapy Research, 9(4): 270-276.  https://doi.org/10.1002/ptr.2650090408

Google Scholar

5. Kirtikar, K.R. and Basu, B.D. (1975). Indian Medicinal Plants. 3: 1647-1649.

Google Scholar

6. Kumar, S.V. and Manoranjan, B. (1992). Forestry Seed Manual of Andhra Pradesh. R & D Circle, A.P. Forest Department, Hyderabad. p. 90.

Google Scholar

7. Mathur, I. and Kumar, M. (1992). Micropropagation of Bauhinea variegata and Parkinsonia aculeata from nodal explants of mature trees. Plant Cell Tiss. Org. Cult., 28: 119-121.  https://doi.org/10.1007/BF00039924

Google Scholar

8. Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant, 15: 473-497.  https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Google Scholar

9. Nandakarni, K.M. (1976). The Indian Materia Medica, 1: 1181-1182.

10. Praveen, M. (2001). In vitro studies on medicinally important plant - Strychnos potatorum Linn.f. M.Tech Dissertation, Jawaharlal Nehru Technological University, pp.24.

11. Praveen, M.; Lakshman, A. and Ramaswamy, N. (2001 a). Callusing efficiency and plant regeneration from different explants of Strychnos potatorum Linn.f - A medicinally important forest tree. Proceedings in Natl. Sem. in Plant Biotechnology : 146-151.

Google Scholar

12. Praveen, M.; Lakshman, A. and Ramaswamy, N. (2001 b). Production of hairy roots in two species of Strychnos using Agrobacterium rhizogenes. Proc. Natl. Conf. Plant Biotechnology : 85-89.

13. Raguramulu, D.; Sri Rama Murthy and Pullaiah, T. (1998). Polyembryony in Strychnos potatorum L. J. Non-timber Forest Prod., 5(1/2): 56-57.

14. Suchitra, B.; Mehar, Z. and Sushil, K. (1999). In vitro multiplication of Centella asiatica, a medicinal herb from leaf explants. Curr. Sci., 76(2): 147-148.

Google Scholar

15. Yashodhara, V. (1998). In vitro Studies on a Medicinal Forest Tree – Strychnos nux-vomica L. Ph.D. thesis, Kakatiya University, pp. 54.

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How to cite

Praveen, M., Lakshman, A., Yugandhar, T. and Ramaswamy, N., 2003. In vitro multiplication of Strychnos potatorum Linn.f. from root explants - A medicinally important plant. Journal of Non-Timber Forest Products, 10(3/4), pp.230-234. https://doi.org/10.54207/bsmps2000-2003-X76JBE

Publication History

Manuscript Published on 01 December 2003

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