Invited Commentary | Published: 31 December 2020

Organogenesis optimization in Dendrocalamus asper

Bhaskar Jyoti Dey, Ashok Kumar Verma, Salil Kumar Tewari and Ashutosh Dubey

Journal of Non-Timber Forest Products | Volume: 27 | Issue: 2 | Page No. 74-77 | 2020
DOI: https://doi.org/10.54207/bsmps2000-2020-ZD4XVN | Cite this article

Abstract

Plant tissue culture offers the potential for rapidly increasing selected bamboo clones for conservation and reforestation. Bud break is important for the successful micro propagation in bamboo. An organogenesis optimization protocol is described for Dendrocalamus asper. Nodal explants containing auxiliary buds from 8-10 years old field grown clumps of D. asper were established in Murashige and Skoog’s (MS) medium supplemented with different concentrations of phytohormone 6-benzyelamino purine (BAP) and kinetin.  The maximum bud breaking was observed in 6 ppm  BAP concentration. Shoot proliferation was found maximum in MS media supplemented with combinations of BAP and kinetin (4.0 ppm  BAP, 0.5 ppm  kinetin and 4.0 ppm  BAP, 1.0 ppm  kinetin) after subcultureing the explants.

Keywords

Bamboo, Dendrocalamus asper, In vitro bud-break, Micropropagation

Access Options

250/-

Buy Full Access in HTML Format

Instant access to the full article.

Get access to the full version of this article. Buy Full Access in HTML Format

References

1. Alexander, M.P. and Rao, T.C., 1968. In vitro culture of bamboo embryo.  Curr. Sci., 37(14), p.415

Google Scholar

2. Arya, I.D., Satsangi, R. and Arya, S., 2002. Rapid Micropropagation of Edible Bamboo Dendrocalamus asper. Journal of Sustainable Forestry, 14(2-3), pp.103-114

Google Scholar

3. Bag, N., Chandra, S., Palani, L.M.S. and Nandi, K., 2000. Micropropagation of Dev-ringal [Thamnocalamus spathiflorus (Trin.) Munro]- a temperate bamboo, and comparison between in vitro propagated plants and seeding. Plant Sci, 156(2), pp.125-135 https://doi.org/10.1016/S0168-9452(00)00212-0

Google Scholar

4. Bangerth, F., 1994. Response of cytokinin concentration in xylem exudates of bean (Phaseolus vulgaris L.) plants to decapitation and auxin treatment, and relationship to apical dominance. Planta, 194, pp.439-442 https://doi.org/10.1007/BF00197546

Google Scholar

5. Bhojwani, S.S., Mullins, K. and Cohen, D., 1984. Intra-varietal variation for in vitro plant regeneration in genus Trifolium. Euphytica, 33, pp.915-921 https://doi.org/10.1007/BF00021921

Google Scholar

6. Das, P. and Rout, G.R., 1994. ‘Analysis of current methods and approaches on the micropropagation of bamboo’, in Proceedings of the National Academy of Sciences India. Biological Sciences, 64(3), pp.235-246

Google Scholar

7. Dransfield, S. and Widjaja, E.A. (eds.), 1995. Plant Resources of South-East Asia No. 7. Bamboos. Backhuys Publishers, Leiden, the Netherlands

Google Scholar

8. Gielis, J. and Oprins, J., 2002. Micropropagation of temperate and tropical woody bamboos from biotechnological dream to commercial reality. Bamboo for Sustainable Development. INBAR Proceedings, VSP Publishers, pp.333-344

Google Scholar

9. Johan, G. and Jan, O., 2001. ‘Micropropagation of temperate and tropical woody bamboos - from biotechnological dream to commercial reality ‘, in Azmy Hj. Mohamed, Ismail Hj. Parlan, Shamsudin Ibrahim, Safiah Yusmah, M. Y., Lim HinFui, Muhammed Azmi, M. I., Ab. Rasip, A.G.,Salmiah Ujang, Khali Aziz Hamzah (eds.), Tropical forestry research in the new millennium: meeting demands and challenges. Proceedings of The International Conference on Forestry and Forest Products Research (CFFPR 2001) held in Kuala Lumpar, Malaysia on 1-3 October 2001, pp.454-462

Google Scholar

10. John, C.K., Joshi, M.S., Nadgauda, R.S. and Mascarenhas, A.F., 1994. Selection - A valuable method for bamboo improvement. Curr. Sci., 66, pp.822-824

Google Scholar

11. John, C.K., Nadgauda, R.S. and Mascarenhas, A.F., 1994. Reproductive biology: An aid in the classification of bamboos. Curr. Sci., 67, pp.685-­687

Google Scholar

12. Marulanda, M.L., Carvajalino, M., Vargas C. and Londono X., 2002. ‘La biotechnologia aplicada al studio y aprovechamiento de la Guadua’,in Seminario-Taller Avances en la Investigacion sobre Guadua, Pereira, Columbia, pp.1-5

13. Meei-ling Yeh and Wei-chin Chang, 1986. Plant regeneration through somatic embrygenesis in callus culture of green bamboo (Bambusa oldhamii Munro). Theor. Appl. Gent., 73, pp.161-163 https://doi.org/10.1007/BF00289269

Google Scholar

14. Murashige T. and Skoog F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant, 15, pp.473–497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Google Scholar

15. Prutpongse P. and Gavinlertvatana P., 1992. In vitro micropropagation of 54 species from 15 genera of bamboo. HortScience, 27, pp.453-454 https://doi.org/10.21273/HORTSCI.27.5.453

Google Scholar

16. Ramanayake S.M.S.D and Yakandawala K., 1997. Microprogation of the giant bamboo (Dendrocalamus giganteus Munro) from nodal explants of field grown culms. Plant Sci, 129, pp.213-223 https://doi.org/10.1016/S0168-9452(97)00185-4

Google Scholar

17. Rao I.U., Rao I.V.R. and Narang V., 1985. Somatic embryogenesis and regeneration of plants in bamboo Dendrocalamus stricus. Plant Cell Rep., 4, pp.191-194 https://doi.org/10.1007/BF00269286

Google Scholar

18. Rao, I.V.R., Yusoff, A.M., Rao, A.N. and Sastry, C.B., 2003. Propagation of bamboo and rattan through tissue culture. Published by The IDRC Bamboo and Rattan Research Network

Google Scholar

19. Ravikumar R., Ananthakrishnan G. Kathiravan K. and Ganapathi A., 1998. In vitro shoot propagation of Dendrocalamus strictus Nees. Plant Cell Tiss Org Cult 52, pp.189-192 https://doi.org/10.1023/A:1006092620731

Google Scholar

20. Templeton-Somers, K.M. and Collins W.W., 1986. Heritability of regeneration in sweet potato (Ipomea batatas). Theor. Appl. Genet., 71, pp.835-841 https://doi.org/10.1007/BF00276426

Google Scholar

21. Thomas T.H. and Blakesley D., 1987. Practical and potential uses of cytokinins in agriculture and horticulture. Br Plant growth Regul Group Monogr, 14, pp.69-83

Google Scholar

22. Tseng, T.C., Lin, D.F. and Shaio, S.Y., 1975. Isolation of protoplast from crop plants. Bot. Bull. Acad. Sinica. 16, pp.55-60

23. Wang, J.W., and Jiang, J.,1998. Study on in vitro culture of bamboo. Forest Research., 11, pp.640-646

24. Woods, S.H., Philips G.C., Woods J.E. and Collins G.B., 1992. Somatic embrygenesis and plant regeneration from zygotic embryo explants in maxican weeping bamboo, Otatea acuminate aztecorum. Plant Cell rep., 11, pp.257-261 https://doi.org/10.1007/BF00235077

25. Zaerr J.B. and Mapes M.O., 1982. Action of growth regulators. In: Bonga J.M. and Durzan D.J. (eds.), Tissue culture in forestry. Martinus Nijhoff/Dr. W.Jun, The Hague, The Netherlands, pp.231-255

Google Scholar

About this article

How to cite

Dey, B.J., Verma, A.K., Tewari, S.K. and Dubey, A., 2020. Organogenesis optimization in Dendrocalamus asper. Journal of Non-Timber Forest Products, 27(2), pp.74-77. https://doi.org/10.54207/bsmps2000-2020-ZD4XVN

Publication History

Manuscript Received on 30 April 2020

Manuscript Revised on 08 August 2020

Manuscript Accepted on 18 August 2020

Manuscript Published on 31 December 2020

Share this article

Anyone you share the following link with will be able to read this content: